Reproducibility Passport · Splice-Blocking Morpholino Knockdown of tbx5a in Zebrafish Embryos with RT-PCR Validation and Fin/Heart Phenotyping — Olto Discovery
Completeness score: 51 out of 100, grade D
D
51/100
Reproducibility Passport
Splice-Blocking Morpholino Knockdown of tbx5a in Zebrafish Embryos with RT-PCR Validation and Fin/Heart Phenotyping
Grade D · Early-stageGenetics
A deterministic, publicly-verifiable summary of how reproducible and transparent this protocol is, computed from open signals only and never from private lab data.
Reproducibility dimensions
Methodological rigor
86
6 of 7 reproducibility signals present.
Passed:
Experimental controlsControls are specified for valid comparison.
Passed:
ReplicationBiological/technical replicates are described.
Not yet met:
Sample size / powerNo sample-size justification — a power analysis or stated n strengthens the design.
Passed:
Statistical analysisA statistical analysis plan is included.
How to strengthen this passport
No sample-size justification — a power analysis or stated n strengthens the design.
Reference a recognized standard your method follows (e.g. ISO 17025, ASTM, USP, 21 CFR Part 11).
Add a references section citing the sources your method builds on.
Link the author ORCID iD for verifiable attribution.
Iterate and version the protocol — a revision history strengthens provenance.
Verifiable artifact
This passport is reproducible: anyone can recompute it from the public protocol. Cite it with the canonical URL below.
Splice-Blocking Morpholino Knockdown of tbx5a in Zebrafish Embryos with RT-PCR Validation and Fin/Heart Phenotyping [Reproducibility Passport]. (2026). Olto Discovery. https://www.oltodiscovery.com/passport/splice-blocking-morpholino-knockdown-of-tbx5a-in-zebrafish-af69bd