Reproducibility Passport · Preparation and Maintenance of Primary Embryonic Rat Hippocampal Neuron Cultures (E18) on Poly-D-Lysine for Long-Term Synaptic Studies — Olto Discovery
Completeness score: 46 out of 100, grade D
D
46/100
Reproducibility Passport
Preparation and Maintenance of Primary Embryonic Rat Hippocampal Neuron Cultures (E18) on Poly-D-Lysine for Long-Term Synaptic Studies
Grade D · Early-stageNeuroscience
A deterministic, publicly-verifiable summary of how reproducible and transparent this protocol is, computed from open signals only and never from private lab data.
Reproducibility dimensions
Methodological rigor
71
5 of 7 reproducibility signals present.
Passed:
Experimental controlsControls are specified for valid comparison.
Passed:
ReplicationBiological/technical replicates are described.
Not yet met:
Sample size / powerNo sample-size justification — a power analysis or stated n strengthens the design.
Passed:
Statistical analysisA statistical analysis plan is included.
How to strengthen this passport
No sample-size justification — a power analysis or stated n strengthens the design.
If your design assigns subjects to groups or scores outcomes subjectively, add randomization/blinding to reduce bias.
Reference a recognized standard your method follows (e.g. ISO 17025, ASTM, USP, 21 CFR Part 11).
Add a references section citing the sources your method builds on.
Link the author ORCID iD for verifiable attribution.
Iterate and version the protocol — a revision history strengthens provenance.
Verifiable artifact
This passport is reproducible: anyone can recompute it from the public protocol. Cite it with the canonical URL below.
Preparation and Maintenance of Primary Embryonic Rat Hippocampal Neuron Cultures (E18) on Poly-D-Lysine for Long-Term Synaptic Studies [Reproducibility Passport]. (2026). Olto Discovery. https://www.oltodiscovery.com/passport/preparation-and-maintenance-of-primary-embryonic-rat-hippo-28abc0