Establish absolute quantification of SARS-CoV-2 N-gene genomic RNA copies in infected Vero E6 culture supernatants by one-step RT-qPCR against an in vitro-transcribed RNA standard curve, and distinguish active replication via subgenomic E-gene RNA detection.
Determine the infectious titer (TCID50/mL) of an influenza A/Puerto Rico/8/1934 (H1N1) virus stock by endpoint dilution on MDCK cells, scoring cytopathic effect to quantify replication-competent virus.
Determine the infectious titer (PFU/mL) of a vesicular stomatitis virus (VSV) stock by counting discrete plaques formed under a semi-solid Avicel overlay on BHK-21 monolayers, providing a direct single-particle infectivity measurement.
Quantify the neutralizing potency (IC50/NT50) of serum or monoclonal antibodies against SARS-CoV-2 spike using single-cycle lentiviral pseudovirus carrying a firefly luciferase reporter on HEK293T-ACE2 target cells, enabling BSL-2 measurement of neutralization without live virus.
Characterize the replication kinetics of human adenovirus type 5 (HAdV-5) in A549 cells over a synchronized low-MOI infection by measuring intracellular and released viral genome copies (qPCR) and infectious titer (TCID50) at defined time points to define eclipse, exponential, and plateau phases.