Quantify the lifespan-extension effect of daf-2 (insulin/IGF receptor) knockdown in C. elegans using bacterial RNAi-by-feeding and rigorous Kaplan-Meier survival analysis, with FUdR to prevent progeny and an empty-vector RNAi control.
Identify dominant modifiers of a rough-eye phenotype by crossing a GMR-GAL4 sensitized-eye line to UAS-RNAi lines and quantifying eye degeneration through pseudopupil counts and ommatidial morphometrics.
Determine whether an uncharacterized recessive lethal mutation (l(2)*) fails to complement a defined deficiency on chromosome 2 by performing balancer-based complementation crosses in Drosophila melanogaster and scoring adult progeny class ratios against Mendelian expectation.
Determine the loss-of-function phenotype of tbx5a in zebrafish by transiently knocking down its splicing with an antisense morpholino oligonucleotide, validating mis-splicing by RT-PCR, and scoring pectoral fin and cardiac defects at 48-72 hpf.
Generate high-efficiency biallelic F0 'crispant' knockouts of slc24a5 in zebrafish by co-injecting multiple synthetic gRNAs with Cas9 protein, and quantify editing efficiency by heteroduplex mobility assay alongside the predicted golden/hypopigmentation phenotype.