Establish a reproducible 1 L batch fermentation of IPTG-induced GFP-expressing E. coli BL21(DE3), quantifying biomass, soluble protein titer, and specific yield against glucose consumption.
Measure and rank the relative transcriptional strengths of a synthetic constitutive promoter library in E. coli using sfGFP fluorescence normalized to a reference standard (relative promoter units, RPU).
Provide a rigorous workflow to verify the correct assembly and sequence integrity of Golden Gate-assembled plasmid constructs in E. coli using both Sanger sequencing of junctions and whole-plasmid Nanopore sequencing.
Quantitatively calibrate the dynamic range, Kd, and reversibility of the eCALWY-4 FRET Zn2+ sensor in live HEK293T cells so it can be used to report cytosolic free zinc with defined units.
Develop and characterize covalent immobilization of lipase B (CALB) on amino-functionalized silica via glutaraldehyde, quantifying immobilization yield, retained specific activity, and operational stability over repeated reaction cycles.